Biomedical Science

Specialized Microscopy

Integrated course, 1.50 ECTS

 

Course content

Advanced physical foundations of microscopy, mechanical and optical parts of a microscope and their function, correct adjustment, sources of error, care and maintenance, optical foundations of image creation, application of relevant microscopic procedures, contrasting techniques , fluorescence microscopy, laser scanning microscopy, electron microscopy.

Learning outcomes

The students know the fundamentals of light microscopy in biomedical science. They can assign different technologies of light microscopy to investigation material under supervision and can carry out these methods independently. The students know the basics of fluorescence microscopy and their areas of application in diagnosis and research. They understand the theoretical basics of electron microscopy and high-resolution microscopy.

Recommended or required reading and other learning resources / tools

  • Alberts et al. (2017): Molekularbiologie der Zelle. 6. Auflage
  • Haus (2014): Optische Mikroskopie
  • Hillenkamp (2002): Mikroskopie
  • Kühl (2018) Grundlagen der Licht- und Elektronenmikroskopie
  • Schmitz und Desel (2018) Der Experimentator Zellbiologie

Bücher, Fachzeitschriften und Internetseiten entsprechend dem aktuellen Wissenstand im Fachgebiet.

Auswahl Fachzeitschriftenartikel:

  • So PT (2002) Two-photon Fluorescence Light Microscopy. Encycl Life Sci. doi:10.1038/npg.els.0002991.
  • Svoboda K, Yasuda R (2006) Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience. Neuron 50(6):823–839.
  • Mattheyses AL, Simon SM, Rappoport JZ (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist. J Cell Sci 123(21):3621–3628.
  • Göttfert F, et al. (2013) Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophys J 105(1):6–8.
  • Betzig E, et al. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313(5793):1642–1645.
  • Kanda T, Sullivan KF, Wahl GM (1998) Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 8(7):377–385.

 

Mode of delivery

integrated course

Prerequisites and co-requisites

Physics on Matura level

Assessment methods and criteria

continuous assessment