Biomedical Science

Analytic molecular biology 2

Integrated course, 2.00 ECTS

 

Course content

Preanalytics, isolation and qualitative and quantitative analysis of nucleic acids, carrying out relevant methods of molecular biology and genetics, including genetic engineering methods on pro- and eukaryotic cell systems, their analysis, interpretation, quality control and bioinformatic analysis in particular with regard to research questions.
Research strategies, primer design and lab-exercises for recombinant DNA (cloning with restrition and ligation; cloning with seamless cloning). Research strategies, primer design and lab-exercises for expression analysis of mRNA in eukaryotic cells.

Learning outcomes

The students know the principles of the research relevant methods and can discuss the application of the suitable tools for solving a problem.  They know the basics of primer design for molecular applications. They can purify nucleic acids and identify possible hazards in preanalytics. They can apply methods of molecular biology, cytogenetics or gene technology when dealing with an experimental research question and carry them out independently and are able to assess them.

Recommended or required reading and other learning resources / tools

  • Alberts, B., Johnson, A., Lewis, J., Morgan, D., Raff, M., Roberts, K., et al. (2017). Molekularbiologie der Zelle. 6th ed. Wiley-VCH.
  • Buckingham, L. (2019). Molecular diagnostics: fundamentals, methods and clinical applications. 3rd ed. Philadelphia: F.A. Davis.
  • Mülhardt, C. (2013). Der Experimentator: Molekularbiologie / Genomics. 7th ed. Springer Spektrum.
  • Reinard, T. (2018). Molekularbiologische Methoden 2.0,. 2nd ed. Stuttgart: Verlag Eugen Ulmer.
  • Strachan, T., and Read, A. P. (2015). Human Molecular Genetics. 5th ed. Garland Science/Taylor & Francis Group.
  • Thiemann, F. (2015). Molekulare Diagnostik: Grundlagen der Molekularbiologie, Genetik und Analytik. 2nd ed. Wiley-VCH.
  • Warford, A., and Presneau, N. (2019). Molecular Diagnostics: Fundamentals of Biomedical Science. Oxford University Press.
  • Zlatanova, J. S. (2016). Molecular Biology: Structure and Dynamics of Genomes and Proteomes. Taylor & Francis.
  • Bücher, Fachzeitschriften und Internetseiten entsprechend dem aktuellen Wissenstand im Fachgebiet.

Auswahl Fachzeitschriftenartikel:
  • Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., et al. (2009). The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55, 611–622.
  • Bustin, S. A., and Nolan, T. (2004). Pitfalls of quantitative real- time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15, 155–166.
  • Gibson, D. G., Young, L., Chuang, R., Venter, J. C., Iii, C. A. H., and Smith, H. O. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–345.
  • Kibbe, W. A. (2007). OligoCalc: An online oligonucleotide properties calculator. Nucleic Acids Res. 35, 43–46.
  • Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408.
  • Nolan, T., Hands, R. E., and Bustin, S. A. (2006). Quantification of mRNA using real-time RT-PCR. Nat. Protoc. 1, 1559–1582.
  • Schmittgen, T. D., and Livak, K. J. (2008). Analyzing real-time PCR data by the comparative C(T) method. Nat. Protoc. 3, 1101–1108.

Mode of delivery

integrated course

Prerequisites and co-requisites

medical bases und molecular biology

Assessment methods and criteria

continuous assessment