Mass Spectrometry and Molecular Analysis

Introdcution into molecular biology (for bachelor graduates with a chemical/technical focus; synchronising molecular biology)

Lecture, 2.00 ECTS

 

Course content

Structure of prokaryotes and eukaryotes, structure and function of macromolecules (proteins, DNA, RNA, carbohydrates), genes, gene structure, chromosomes, information flow from gene to protein, DNA replication, transcription and translation, genetic codes, tools of molecular biology (bacteria, viruses, enzymes) vectors, synthetic oligonucleotides. Standard methods in molecular biology: Extraction of nucleic acids, determination of concentrations, detection of nucleic acids (blotting procedure), electrophoretic separation (nucleic acids and proteins), reverse transcription, PCR, blotting procedures, cloning, sequence analysis, foundations and applications of human and cytogenetics (mutations, heredity, diagnostics)

Learning outcomes

As a preparation for the following core modules and modules of both the areas of specialisation, the students will be taught, depending on prior knowledge, in two introductory components in chemistry and molecular techniques. In both areas basic knowledge from bachelor studies will be revived, interconnected to higher cognitive levels to enable a broad competence network necessary for understanding and performing modern bioanalytical and molecular applications.
The introductory courses in chemistry focus on stoichiometry, physical chemistry and organic chemistry. The students can perform serial dilutions and buffer solutions for a given problem and identify problems associated with these tasks. The students are able to form a chemical reaction equation, to parameterize them correctly, to carry out the appropriate calculations and to suggest consequences of concentration changes and other parameters on reaction balance. Students can discuss chemical bindings in organic molecules, can assign functional groups to characteristic reactions, can apply important types of reaction and can designate reaction products including their stereochemistry. In the area of biological foundations the students understand the structure of prokaryotic and eukaryotic cells both from the chemical and biological view, can assign suprameolecular structures and cell organelles to their function, can describe the mechanisms of important cellular processes (cell division, membrane transport, metabolism) and identify relevant interactions and possible problems. Based on the central dogmas of molecular cell biology and genetics the students can analyze gene expression regulation and suggest methods for studying gene expression changes. The students can plan basic gene and molecular biological technologies and suggest relevant quality control measures.