Mass Spectrometry and Molecular Analysis

Special chapters of mass spectrometry

Seminar, 4.00 ECTS

 

Course content

Theoretical background of mass analyzers
Combination of ionizing techniques and mass analyzers
Application of common MS systems
High-resolution mass spectrometry
Database supported identification of spectra
Identification of unknown samples in MS
Mass spectra of different classes of compounds
Detailed mechanisms of ion fragmentation
Potential and challenges of data processing
Detailed sample preparation
Potential of derivatization techniques
MALDI-MS and SIMS-MS imaging
Development of solution strategies with MS for problems in biology

Learning outcomes

The students are able to deal with special questions in mass spectrometry (MS). They have in-depth knowledge of the function of different MS systems and their range of applications, like time of flight mass spectrometry (TOF), linear quadrupol analyzers, linear and three dimensional quadrupol traps, orbitrap analyzers and Fourier transform ion cyclotron resonance systems. They are familiar with different molecular ionizing techniques and can choose the appropriate method specific to the situation (e.g. electron impact ionization, chemical ionization, field ionization and field resorption, fast atom bombardment, matrix-supported laser desorption/ionization and electrospray ionization). The students know the current technologies of the combination of chromatography and mass spectrometry procedures and are able to plan and carry out appropriate experiments for GC/MS and LC/MS systems. They are familiar with the special technologies of selected ion monitoring and selected reaction monitoring in connection with concepts of chromatography/mass spectrometry. They master techniques of quantification and their standardizing procedures, like internal and external standards and isotope dilution techniques. In the field of chromatographic separation methods the students acquire in-depth knowledge/comprehensive knowledge for collecting and preparing specimens. This includes the specific concentration of analytes with solid-phase extraction (SPE), the application of appropriate centrifugation techniques and goal-oriented derivatisation techniques. In addition, the students know how to optinmize chromatographic procedures with professional methods to efficiently and effectively adapt the analysis protocols with regard to the choice of appropriate isocratic liquid phases or complex solvent gradients and the choice of optimal temperatures and appropriate separation columns.