Mass Spectrometry and Molecular Analysis

Chromatographical and spectrometrical techniques

Tutorial, 4.00 ECTS

 

Course content

putting theory into practice: instrumental main component and trace analytics:
- creation of an analysis plan
- production of standard solutions and serial dilutions
- measuring of standard samples with chromatographical and spectrometrical techniques
- creation of standard curves
- analysis of a sample containing an unknown amount of a well-known analyte
- sample collection
- sample preparation
- separation of complex mixtures with LC and GC
- interpretation of the data
- evaluation and logging

In addition to handling of single procedures the focus is placed on the holistic aspect of the analytical procedure

Learning outcomes

The students understand the fundamental mechanisms and formal relationships between chromatographic, electrophoretic and spectrometric techniques. In the field of chromatographic methods they can characterize chromatographic processes with regard to the classification of liquid, gas, gas/liquid, thin-layer chromatography (LC, GC, GLC and DC) and capillary electrochromatography (CE). They know the basic technical structure of LC and GC systems and can describe the individual function modules. According to the separation criteria they are able to differentiate between normal phase (NP - LC), reversed phase (RP - LC), ion exchange (IEX- LC), size-exclusion chromatography (SEC - LC) and hydrophilic interaction chromatography (HILIC) and can apply them goal-oriented. They can successfully apply criteria for the choice of carrier materials and the associated separation method. In GC and GLC the students can explain the fundamental principle of separation of analytes and can choose an appropriate separation column and the associated parameter regime. In electrophoretic procedures, the students can explain the physical principles with regard to the separation criteria of molecular weight and the associated hydrodynamic factor and the charging of analytes and can choose a suitable technology in a specific situation. They know about native and denaturing methods and are able to define suitable separation conditions with regard to the choice of the required puffer systems and the associated pH value ranges. In the field of spectrometric procedures and techniques the students develop competence in the theory of UV/VIS IR-, Raman-, fluorescence- and mass spectrometry. They can differentiate between electron excitation spectra and molecular vibration spectra. They are familiar with the principle of fragmentation of molecules in mass spectrometry. They are able to to choose the suitable procedure of a given task and to plan and carry out the analytical processes. They know how to interpret appropriate spectra, and, thus, can evaluate and interpret the results of their analyses.