Mass Spectrometry and Molecular Analysis

Molecular and mass spectrometry

Lecture, 3.00 ECTS

 

Course content

UV/VIS spectrometry
- principles of UV/VIS spectroscopy
- principle structure and function of a UV/VIS spectrometer
- chromophores and auxochromes
- spectroscopical phenomena of chemical compounds
- structural analysis
Infrared and Raman spectrometry
- principles of IR spectroscopy
- principle structure and function of an IR and Raman spectrometer
- characteristic vibrational spectra of chemical compounds
- structural analysis
Mass spectrometry
- principles of mass spectrometry
- principle structure and function of a mass spectrometer
- techniques of ionisation of molecules and source design
- main fragmentation reaction of organic compounds
- performance of different technologies and their scope of application

Learning outcomes

The students understand the fundamental mechanisms and formal relationships between chromatographic, electrophoretic and spectrometric techniques. In the field of chromatographic methods they can characterize chromatographic processes with regard to the classification of liquid, gas, gas/liquid, thin-layer chromatography (LC, GC, GLC and DC) and capillary electrochromatography (CE). They know the basic technical structure of LC and GC systems and can describe the individual function modules. According to the separation criteria they are able to differentiate between normal phase (NP - LC), reversed phase (RP - LC), ion exchange (IEX- LC), size-exclusion chromatography (SEC - LC) and hydrophilic interaction chromatography (HILIC) and can apply them goal-oriented. They can successfully apply criteria for the choice of carrier materials and the associated separation method. In GC and GLC the students can explain the fundamental principle of separation of analytes and can choose an appropriate separation column and the associated parameter regime. In electrophoretic procedures, the students can explain the physical principles with regard to the separation criteria of molecular weight and the associated hydrodynamic factor and the charging of analytes and can choose a suitable technology in a specific situation. They know about native and denaturing methods and are able to define suitable separation conditions with regard to the choice of the required puffer systems and the associated pH value ranges. In the field of spectrometric procedures and techniques the students develop competence in the theory of UV/VIS IR-, Raman-, fluorescence- and mass spectrometry. They can differentiate between electron excitation spectra and molecular vibration spectra. They are familiar with the principle of fragmentation of molecules in mass spectrometry. They are able to to choose the suitable procedure of a given task and to plan and carry out the analytical processes. They know how to interpret appropriate spectra, and, thus, can evaluate and interpret the results of their analyses.