Mass Spectrometry and Molecular Analysis

Methods of molecular biology and gene technology

Integrated course, 5.00 ECTS

 

Course content

Isolation of nucleic acid (e.g. from complex sample matrices like soil samples, waste water, breath and plasma), amplification and detection of nucleic acids by using different detection formats (e.g. digital PCR, qPCR, in situ…) selection of suitable vector and host systems, examination and influencing of gene regulation (real-time PCR, RNA interference,...) mutagenesis, high-throughput technologies (generation of libraries, next generation sequencing, RNAseq, micro-arrays (oligonucleotide, peptide arrays), ChIP-Seq, methylation analysis), protein expression systems, in situ detection of proteins, principles of protein design; criteria for choice of method; selection of the most suitable method for a concrete question (e.g. forensic science, waste water monitoring, antibody production).

Learning outcomes

(1) The students are able to describe the advantages and disadavatages of methods of molecular techniques ( above all in the fields of molecular diagnostics, nanotechnology, OMICS- technologies, molecular biological and gene technological applications.) (2) They can choose the appropriate question- specific molecular techniques, plan and carry out additional experiements, evaluate, validate and interpret the results. (3) They are able to discuss and apply measures for quality assurance. (4) They can plan and discuss developmental steps in the production of recombinant proteins (medications, vaccines, food additives).