Department of Health Studies

Mass Spectrometry and Molecular Analysis

My Studies

 
Curriculum

1. Semester

Bioinformatics and statistical procedures | Integrated course (iL) | Coursecode: 150754103 Statistical procedures, data preparation and data analysis 2 SWS 4 ECTS
Application-oriented statistical foundations, descriptive statistics for the biometrics laboratory, tools and instruments for statistical data analysis and interpretation, quality assurance based on statistical procedures.
Biological and chemical foundations | Integrated course (iL) | Coursecode: 150754101 Chemistry and stoichiometry 5 SWS 7 ECTS
Organic Chemistry:
- Stereochemistry
- Heterocycles
- Structure of biomolecules and their chemical potential
- Chemical bonding in organic molecules
- Classification and properties of substance classes
- Alkanes, alkenes and alkynes
- Cycloalkanes and aromatics
- Important functional groups and their reactions
- Important reaction mechanisms of organic chemistry
- Simple chemical synthesis processes

Physical Chemistry:
- Chemical catalysis
- The Gibbs function and its role in chemical reactions
- Kinetics of chemical reactions
- Theory of chemical bonding
- The laws of thermodynamics

Stoichiometry:
- Calculation of aqueous mixtures
- Calculation of complex kinetic processes
- Calculation of enzyme-catalyzed reactions
- Working with equilibrium constants
- Calculation of acid / base reactions
- The pH concept and calculation of buffer systems
- Calculate of redox reactions
- Calculation of basic kinetic processes
Biological and chemical foundations | Lecture (Vo) | Coursecode: 150754108 Introdcution into molecular biology (for bachelor graduates with a chemical/technical focus; synchronising molecular biology) 2 SWS 2 ECTS
Structure of prokaryotes and eukaryotes, structure and function of macromolecules (proteins, DNA, RNA, carbohydrates), genes, gene structure, chromosomes, information flow from gene to protein, DNA replication, transcription and translation, genetic codes, tools of molecular biology (bacteria, viruses, enzymes) vectors, synthetic oligonucleotides. Standard methods in molecular biology: Extraction of nucleic acids, determination of concentrations, detection of nucleic acids (blotting procedure), electrophoretic separation (nucleic acids and proteins), reverse transcription, PCR, blotting procedures, cloning, sequence analysis, foundations and applications of human and cytogenetics (mutations, heredity, diagnostics)
Biological and chemical foundations | Lecture (Vo) | Coursecode: 150754107 Introduction into chemistry (for bachelor graduates with a biological focus; synchronising chemistry) 2 SWS 2 ECTS
General chemistry:
- basic laws of chemistry
- foundations of the atomic theory and chemical bindings
- the periodic table of elements
- the law of mass action and the chemical equilibrium
- types of chemical reaction (acid-base reactions, radical reactions, redox reactions)
- terminology of chemical bindings

Stoichiometry:
- forming and paramterizing of chemical reaction equations
- calculating chemical reactions
- calculating serial dilutions
- calculating aqueous mixtures
Biological and chemical foundations | Integrated course (iL) | Coursecode: 150754104 Molecular cell biology, genetics and foundations of gene technology 5 SWS 7 ECTS
Legal bases of genetic engineering, differences in bacterial and mammalian genetics, possibilities of gene transfer in prokaryotes and eukaryotes, selection, protein expression systems, cell lysis/cell disruption, genetic regulatory mechanisms, cellular transport systems, protein degradation, cell-cell communication, mutagenesis, classical genetics and advanced genetics by the example of selected host organisms, basics of microbiology and virology, micro-organisms and viruses as tools of genetic engineering, species definition/taxonomy, laboratory animal science, analysis and cross-breeding of mouse lines, the production of transgenic organisms, special features of stem cells, discussion about the above-mentioned contents in specific fields of application
Modultitel Englisch | Integrated course (iL) | Coursecode: 150754102 Chromatographic and electrophoretic separation techniques I 3 SWS 4 ECTS
Foundations of chromatographic techniques:
- Taxonomy of chromatographic procedures
- principles of chromatographic separation
- liquid chromatography
- instrumentation
- important parameters
- Van Deemter equation
- stationary phases
- mobile phases
- reversed-phase chromatography
- normal-Phase chromatography
- ion exchange chromatography
- hydrophilic interaction chromatography
- chiral chromatography
- affinity chromatography
- gel-permeation chromatography
- principles of isocratic separation and gradient separation
- detectors
- Gas chromatography
- instrumentation
- important parameters
- Raoult's law
- separation columns
- carrier gases
- retention mechanisms
- detectors
- principles of temperature dependent separation of volatile and semi-volatile compounds
Foundations of electrophoretical techniques:
- principles of electrophoretical separation
- capillary zone electrophoresis
- instrumentation
- retention mechanisms
- detectors

Selection and application of chromatographic and electrophoretic separation techniques in chemical analysis, theory and practice of opitimization of separation parameters
Scientific methods and research techniques | Seminar (Se) | Coursecode: 150754106 Data bases and effective literature search 1 SWS 2 ECTS
Search strategies for literature and other databases (introduction to relevant databases, selection of search keys, systematic approach, primary sources, scientific relevance, search documentation ); practical approach models for efficient and effective literature management, reference management software.
Scientific methods and research techniques | Seminar (Se) | Coursecode: 150754105 Team management & social media 1 SWS 2 ECTS
Development of attitudes and strategies that aid in increasing team performance and individual satisfaction and reduce conflict when collaborating in a team. The students get to know how to use digital media when working in a team and how to use professionally digital resources of the UAS JOANNEUM (audio/video conferencing, resource sharing, the LMS moodle, VPN access to resources of the university intranet), data protection and legal issues ( UAS JOANNEUM data protection guide).

2. Semester

Bioinformatics and statistical procedures | Integrated course (iL) | Coursecode: 150754209 Applied bioinformatics 2 SWS 4 ECTS
Introduction and use of different data bases (sequence, structure, genome and interaction data bases), prominent web portals (NCBI, ExPASy, KEGG), principles of sequence analysis (alignment, assembly, annotation), homologies, prediction programs (splice sites, promoter, mutations), system theory and systems thinking, simulation techniques, simulation of biological processes and systems, modelling of cellular systems.
Bioinformatics and statistical procedures | Integrated course (iL) | Coursecode: 150754207 Quality assurance and validation in analytics 2 SWS 2 ECTS
Need for quality standards by the Food and Drug Administration (FDA) and the Environmental Protection Agency (EPA); general criteria for operating test laboratories (ÖNORM EN 45001, ISO/IEC 17025); definition, explanation and commenting of quality assurance terminology, prerequisites for the validation of an analytical method, validation parameters and their examination (reproducibility, repeatability, selectivity, sensitivity, recovery, precision, accuracy, detection and quantification limits, robustness), methods to characterize analytical methods, flow chart for method validation, release of method, documentation.
Modultitel Englisch | Tutorial (Ue) | Coursecode: 150754203 Chromatographical and spectrometrical techniques 3 SWS 4 ECTS
Modultitel Englisch | Seminar (Se) | Coursecode: 150754208 Interpretation of spectra 2 SWS 2 ECTS
- model procedures for interpretation of spetra of chemical compounds
- interpretation and discussion of UV/VIS spectra with practical examples
- interpretation and discussion of IR spectra with practical examples
- theory of isotope distribution in mass spectrometry
- advanced molecule-ion theory in mass spectrometry
- priciple mechanisms of ion fragmentation in GC-MS ionization technique
- priciple mechanisms of ion fragmentation in LC-MS ionization technique
- interpretation and discussion of MS spectra with practical examples
- postulation of molecular structure with the combined innterpretation of UV/VIS, IR and MS spectra
Modultitel Englisch | Lecture (Vo) | Coursecode: 150754202 Molecular and mass spectrometry 3 SWS 3 ECTS
UV/VIS spectrometry
- principles of UV/VIS spectroscopy
- principle structure and function of a UV/VIS spectrometer
- chromophores and auxochromes
- spectroscopical phenomena of chemical compounds
- structural analysis
Infrared and Raman spectrometry
- principles of IR spectroscopy
- principle structure and function of an IR and Raman spectrometer
- characteristic vibrational spectra of chemical compounds
- structural analysis
Mass spectrometry
- principles of mass spectrometry
- principle structure and function of a mass spectrometer
- techniques of ionisation of molecules and source design
- main fragmentation reaction of organic compounds
- performance of different technologies and their scope of application
Molecular techniques and life sciences | Integrated course (iL) | Coursecode: 150754204 Applied OMICS technologies (transcriptomics, genomics, proteomics, metabolomics) 3 SWS 4 ECTS
Molecular techniques and life sciences | Integrated course (iL) | Coursecode: 150754206 Medical and environmental molecular diagnostics 1 2 SWS 4 ECTS
Principles and advanced knowledge of molecular diagnostics, diagnostic strategies and procedures, carrier and test systems, biomarkers, criteria for method selection, practical examples in medicine, industry and environment ( detection of genetic diseases, forensic medicine, detection of genetically modified organsims, detection of pathogens in body fluids, drinking water, animal feeds and food); methods with a focus on their application in diagnostics: immunoassays (ELISA, immuno PCR, Westernblot), PCR (real time PCR, digital PCR), mutation analysis (SNP genotyping, DNA fingerprinting), microarray based methods (CGH arrays, SNP arrays, protein and antibody arrays), quality assurance
Molecular techniques and life sciences | Integrated course (iL) | Coursecode: 150754201 Methods of molecular biology and gene technology 3 SWS 5 ECTS
Molecular techniques and life sciences | Lecture (Vo) | Coursecode: 150754205 Nanotechnology 1 SWS 2 ECTS
Nanotechnology in medicine, medical technology and environment: molecular targeting, molecular imaging, drug delivery, surface coating, nanotoxicology, environmental monitoring

3. Semester

Bioinformatics and statistical procedures | Integrated course (iL) | Coursecode: 150754304 Data management, data interpretation and structure analysis 2 SWS 4 ECTS
Biological data management and knowledge management systems, data base structure and data formats, procedures of exploratory data analysis, normalization procedures and data reduction, data and structure analysis with multivariate statistical approaches (principal component analysis (PCA), independent component analysis (ICA), PLS discriminant analysis (PLS-DA), cluster analysis.
Scientific methods and research techniques | Lecture (Vo) | Coursecode: 150754301 Design of experiments 1 SWS 2 ECTS
General and statistical planning of experiments in (pre-) clinical and analytical- technical contexts, experimental strategies and creating experimental designs (2k - fractional factorial designs, full factorial designs, response surface designs), evaluation of experimental data with analysis of variance and /or regression, critical success factors in DOE.
Scientific methods and research techniques | Integrated course (iL) | Coursecode: 150754303 Interdisciplinary research project 2 SWS 4 ECTS
Implementation of an independent project (in team work) with a clear initial research question, information and literature search, designing and conducting appropriate experiments, data evaluation and interpretation of the results, write-up and presentation of the results.
Scientific methods and research techniques | Seminar (Se) | Coursecode: 150754302 Scientific writing and presentation 1 SWS 2 ECTS
Tips and tricks for compiling written research papers (structure, referencing, graphical representation), professional abstract writing, short papers, full papers, posters and presentations in English language.
Field mass spectrometry:
Mass spectrometry | Lecture (Vo) | Coursecode: 150754307 Chromatographic separation techniques II 2 SWS 2 ECTS
Detailed mechanisms of gradient separation and isocratic separation
Special chromatographic extraction and enrichment techniques (solid-phase extraction (SPE), in-tube extraction (ITEX), liquid-liquid extraction (LLE))
Special column materials (carrier materials and ligands) and their application
Mobile phases (mixtures and viscosity)
Nano LC
Injection techniques in gas chromatography
Detailed discussion of applications in chromatography
Mass spectrometry | Tutorial (Ue) | Coursecode: 150754309 Focus-specific project work 1 SWS 6 ECTS
The students work out representative analytical methods according to the given requirements. They cover the complete process from formulating the research question, searching for relevant literature and resources, preparing the samples to carrying out the analytical procedure and evaluating and disseminating the results. Case studies enhance their awareness of the complexity of bioanalytical problems. Thus, the students are enabled to draft independently application oriented solution possibilities to complex analytical problems. In this course the students need to compile and present their own project papers and must answer questions about the analytic process that they have dealt with.
Mass spectrometry | Integrated course (iL) | Coursecode: 150754308 Method development and optimization 2 SWS 2 ECTS
Techniques of sample collection
Stabilizing and storage of samples
Concentration and isolation of analytes from complex matrices
Optimization of sample preparation (choice of suitable solvents, optimizing of pH value and ion strength of a solvent)
Optimization strategies for eluents and stationary phases
Selectivity of columns in chromatography
Optimization of reversed phase chromatography
Optimization of normal phase chromatography
Using principal component analysis (PCA) to evaluate chromatographical procedures
Chemometrical methods for optimizing the chromatographic process
Optimization techniques in gas chromatography
Systematic search for errors in LC-MS and GC-MS
Mass spectrometry | Tutorial (Ue) | Coursecode: 150754306 Practical exercises in mass spectrometry 4 SWS 4 ECTS
Planning of complex analysis processes and choosing the appropriate LC/MS and GC/MS techniques
Preparation of tools and standard samples and calibration of the required analysis systems
Optimization of parameters of the analytic devices that meet the requirements for safe and sensitive detection of targeted analytes
Experimental identification of limit of detection and limit of quantitation of the analytes
Separation of complex substance mixtures using computer-assisted mass spectrometry techniques
Identification of characteristic ions for qualitative and quantitative measurement of analytes
Application of appropriate procedures for method optimization with regard to separation and effective ionization of target analytes
Computer-aided data processing and reporting
Mass spectrometry | Seminar (Se) | Coursecode: 150754305 Special chapters of mass spectrometry 3 SWS 4 ECTS
Theoretical background of mass analyzers
Combination of ionizing techniques and mass analyzers
Application of common MS systems
High-resolution mass spectrometry
Database supported identification of spectra
Identification of unknown samples in MS
Mass spectra of different classes of compounds
Detailed mechanisms of ion fragmentation
Potential and challenges of data processing
Detailed sample preparation
Potential of derivatization techniques
MALDI-MS and SIMS-MS imaging
Development of solution strategies with MS for problems in biology
Field molecular diagnostics, therapy and production:
Molecular diagnostics, therapy and production | Lecture (Vo) | Coursecode: 150754314 Ethics in biomedicine 0.5 SWS 1 ECTS
Ethics in biomedicine (in the fields of gene technology, molecular diagnostics, gene therapy, planning of experimental and clinical studies, stem cells, personalized medicine)
Molecular diagnostics, therapy and production | Integrated course (iL) | Coursecode: 150754311 Gene therapy 2 SWS 3 ECTS
Principles, methods and vectors in somatic gene therapy, advantages and disadvantages of the individual administration form, risks, legal aspects, other innovative therapy strategies like cloning or stem cell therapy, application examples
Molecular diagnostics, therapy and production | Integrated course (iL) | Coursecode: 150754315 Interdisciplinary applications 2 SWS 4 ECTS
functional genomics; multidsiciplinary networking and interdisciplinary practical examples of molecular techniques
Molecular diagnostics, therapy and production | Integrated course (iL) | Coursecode: 150754310 Medical and environmental molecular diagnostics 2 3 SWS 4 ECTS
Criteria for the choice of biomarkers; procedures to identify new biomarkers (medical and environmental); single cell analysis; special knowledge in next generation sequencing, practical application and data evaluation.
Molecular diagnostics, therapy and production | Lecture (Vo) | Coursecode: 150754313 Pharmacogenomics and personalized medicine 1.5 SWS 2 ECTS
Foundations of pharmacogenomics; methods/ background; opportunities and risks of individualized medicine
Molecular diagnostics, therapy and production | Integrated course (iL) | Coursecode: 150754312 Protein design and engineering 3 SWS 4 ECTS
In silico modelling, possibilities of structure change (mutagenseis, synthetic biology, tags, fusion proteins) production systems, practical examples from vaccine development, dietary supplements, medication.

4. Semester

Master thesis | Master Thesis (MA) | Coursecode: 150754401 Master thesis 0 SWS 26 ECTS
The aim of the master thesis is to work out independently an innovative job-specific topic, to examine it scientifically and to record the results in a thesis.
Master thesis | Seminar (Se) | Coursecode: 150754402 Master thesis seminar 2 SWS 4 ECTS